hp-Vector表達(dá)系統(tǒng)megaterium芽孢桿菌
Features:
High performance vectors with optimized sequence
Protein yield up to 10 times enhanced compared to protein expression with basic plasmid
Plasmids with established MCS
Encoding C- or N-terminal His-tag for versatile purification (native, 6xHis-tag)
Secretion with LipA or YocH signal peptide up to 9 fold increased
optimized high performance (hp) vectors for Bacillus megaterium!
MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable to most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence and a 6xHis-tag.
With our new hp-vectors yields up to 10-fold higher compared to the basic plasmids are possible. All plasmids have established multiple cloning sites (MCS) for versatile cloning. Furthermore we offer vectors encoding C- or N-terminal His-tags for easy purification. The protein secretion with LipA or YocH signal peptides is up to nine-fold increased. Induction of protein expression of all vectors is achieved by the tightly regulated and efficiently inducible xylose operon.
Versatile system with a wide range of vectors
The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small- and large-scale.
B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.
B. megaterium protoplasts specifically optimized for transformation
The protoplasts supplied by MoBiTec are from B. megaterium strain WH320, developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.
For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.
Order Information, shipping and storage
Please click on the respective order # for further details.
order# description amount
BMEG02 Bacillus megaterium protoplasts ready for transformation (strain WH320;1)
Material is sufficient for 4 transformations plus control experiment. 5 x 500 µl
BMEG50 Bacillus megaterium protoplasts, strain MS941 5 x 500 µl
BMEG31 pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 μg
BMEG32 pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site 10 μg
BMEG30 p3STOP1623hp, shuttle vector, lyophilized DNA 10 μg
BMEG33 pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg
BMEG34 pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA 10 µg
shipped at RT, protoplasts shipped on dry ice
store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C
All vectors are E. coli / B. megaterium shuttle vectors
Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase and use of protoplasts must be thoroughly planned.
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■其它名稱: | VE-cadherin;cadherin-5;Cdh5;Vascular endothelial cadherin;7B4 antigen;CD144 antigen;VECD;V |
■交叉反應(yīng): | rat, mo |
■產(chǎn)品類型: | 一抗 |
■應(yīng)用領(lǐng)域: | /腫瘤/心血管/免疫學(xué)/信號轉(zhuǎn)導(dǎo)/細(xì)胞粘附分子/血管內(nèi)皮細(xì)胞/上皮細(xì)胞/內(nèi)皮細(xì)胞/細(xì)胞外基質(zhì) |
■蛋白分子: | predicted molecular weight: 85-115kDa |
■濃度值: | 1mg/1ml |
■物理性狀: | Lyophilized or Liquid |
■免疫原: | KLH conjugated synthetic peptide derived from human CD144 C-terminus |
■亞型: | polyclonal |
■純化方法: | affinity purified by Protein A |
■保存液: | 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide |
■應(yīng)用技術(shù): | //WB=1:100-500//ELISA=1:500-1000//IP=1:20-100//IHC-P=1:100-500//IHC-F=1:100-500//IF=1:100-500(石蠟切片需做抗原修復(fù))/not yet tested in other applications./optimal dilutions/concentrations should be determined by the end user. |
■保存環(huán)境: | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
pGEM-T easy Vector (T載體) 1.2ug
土霉素 進(jìn)口分裝 10g 羅紅霉素 進(jìn)口分裝 10g 托普霉素 進(jìn)口分裝 1g EDTA Na2 乙二胺四乙酸二鈉 Amresco 100g EDTA Na2 乙二胺四乙酸二鈉 Amresco 500g EDTA FREE ACLD 乙二胺四乙酸 Amresco 100g EDTA FREE ACLD 乙二胺四乙酸 Amresco 500g EGTA 乙二醇-雙-(2-氨基乙基)四乙酸 Amresco 5g EGTA 乙二醇-雙-(2-氨基乙基)四乙酸 Amresco 10g Ethidium Brmide 溴化乙錠 Amresco 100mg Ethidium Brmide 溴化乙錠 Amresco 1g goldview 自產(chǎn) 1ml Evans Blue 埃文斯藍(lán) Sigma 1g Eosin Y 伊紅Y(醇溶) 進(jìn)口分裝 10g Eosin Y 伊紅Y Amresco 10g Ethephon 乙烯利 國產(chǎn) 25g Fluorescein diacetate二乙酸熒光素 Sigma 1g Fibrinogen 纖維蛋白原 Sigma 100mg Fibrinogen 纖維蛋白原 Sigma 1g Forchior fennron 氯吡苯脲 進(jìn)口分裝 1g Ficoll 400 聚蔗糖400 Pharmacia 10g
全國銷售熱線: 客服熱線:
型號名稱 | 型號代碼 | 描述/選項 |
SDC-H1-08 | 40-30 0060 | 探針長度77mm (3”) |
SDC-H1-16 | 40-30 0061 | 探針長度157mm (6.2”) |
型號名稱 | 型號代碼 | 精度 [%rH] | 精度[K]@25°C (77°F) | 描述/選項 |
AES-HT-A2 | 40-50 0031-2 | 2% | ± 0.5° | 溫濕度敏感元件 |
AES-HT-A3 | 40-50 0031-3 | 3% | ± 0.4° | |
AES-HT-A5 | 40-50 0031-5 | 4.5% | ± 0.3° |
型號名稱 | 型號代碼 | 描述/選項 |
OPC-S | 40-50 0029 | 罩殼顯示 |
OPA-S | 40-50 0006 | 外接顯示 |
AMC-1 | 20-10 0035 | 連接線纜Æ 4 – 8 mm (AWG 6 – 1) |
AMC-2 | 20-10 0067 | 線纜連接頭 NPT 1/2 |
www.huaxiatech.cn
EasyXpress pIX4.0 Vector
Vector試劑
詳細(xì)介紹:產(chǎn)品名稱:
pGEM-T easy Vector (T載體) |
檢測試劑:免疫檢測、基因檢測、化學(xué)檢測
原料試劑:抗原抗體、分子生物、生化試劑北京雅杰寶生物科技有限公司“產(chǎn)品名稱”新品上市,本公司主營產(chǎn)品:elisa試劑盒、生物試劑、血清、抗體、培養(yǎng)基、標(biāo)準(zhǔn)品,,,RD歡迎咨詢。
pAT5 vector價格 規(guī)格:10ug × 5支,產(chǎn)品的價格|使用說明,全國配送方式
公司專業(yè)經(jīng)銷ELISA試劑盒多年,產(chǎn)品質(zhì)量有,現(xiàn)貨銷售elisa試劑盒,培養(yǎng)基,標(biāo)準(zhǔn)品,抗體,生物試劑,生化試劑等試劑產(chǎn)品。供應(yīng)現(xiàn)貨產(chǎn)品,品質(zhì)齊全,請來電咨詢。
pAT5 vector價格 及相關(guān)產(chǎn)品庫。
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